CeBEM Infrastructure

Infrastructure Available for Carrying out the Project

The equipment required to do Structural Biology is frequently expensive, some instruments being highly sophisticated.

This is why improved access to the several technological facilities available within CeBEM, mean a better way for students and researchers to perform cutting-edge experiments.

CeBEM nodes functioning at the several institutions that constitute CeBEM, possess equipment to perform Structural Biology, which has been purchased and installed with national funding support. Global investment figures for the whole ensemble are in the order of >20 millions u$s.

São Carlos
* 3 X-ray diffractometers for proteins with rotating anode sources and area detectors
* Crystallization robot
* Biophysical techniques: CD, fluorescence, EPR, stopped-flow, Biacore, SAXS
* Microcalorimetry (DSC, ITC)
* Extensive protein chemistry, molecular biology, cell culture and genome sequencing facilities
* Extensive set of medicinal chemistry software
* Dedicated bioinformatics computers, software for modeling / simulation

Rio de Janeiro
* 3 NMR spectrometers for proteins – 800 MHz, 600 MHz and 400 MHz
* Triple resonance cryoprobe for the 600 MHz machine
* Biophysical techniques: CD, fluorescence, EPR, stopped-flow, Biacore
* Spectrometer for fluorescence lifetime measurements
* Microcalorimetry (DSC, ITC)
* Confocal optical microscopy and fluorescence correlation spectroscopy
* Scanning electron microscopy
* Cluster of computers for calculation of structures and molecular dynamics simulations
* Extensive protein chemistry, molecular biology, cell culture and genome sequencing facilities

Buenos Aires
@ the Leloir Institute Foundation:
* 1 X-ray diffractometer for proteins with a rotating anode source and area detector
* 600 MHz NMR with cryo probe
* Crystallization robot
* Biophysical techniques: CD, fluorescence, EPR, stopped-flow, DLS, SPR, SLS
* Microcalorimetry (ITC)
* Extensive protein chemistry, molecular biology, cell culture and genome sequencing facilities

@ the School of Sciences, University of Buenos Aires:
* Dedicated high performance cluster for computer simulation.
* Software for molecular modeling (classical, quantum mechanical, and hybrid quantum-classical simulations).
* Software for bioinformatics.
* Resonant Raman equipment.
* Biophysical techniques: stopped flow, flash photolysis.

Rosario
* 1 X-ray diffractometer for proteins with a rotating anode source and area detector
* 600 MHz NMR
* Biophysical techniques: CD, fluorescence, EPR, stopped-flow
* Microcalorimetry (ITC)
* Extensive protein chemistry, molecular biology, cell culture and genome sequencing facilities
* Comprehensive suite of software capabilities in medicinal chemistry and chemical synthesis

Montevideo:
@ the Pasteur Institute of Montevideo:
* 1 X-ray diffractometer for proteins with a rotating anode source and area detector
* Crystallization robot and semiautomated crystal visualization instrumentats.
* Biophysical techniques: fluorescence (fluorometer, fluorescence anisotropy), Biacore, DLS
* Microcalorimetry (ITC, DSC)
* Extensive protein chemistry, MS (MALDI-TOF, TOF-TOF, ESI), molecular biology, cell culture and genome sequencing facilities
* Dedicated computer cluster for bioinformatics, software for molecular modeling / simulation
* Recombinant protein production and purification systems (prokaryotic and eukaryotic systems)
* Confocal optical microscopy / fluorescence microscopy.

@ the Biomedical Research Center:
* Two stopped-flow spectrophotometers/fluorometers
* Electron paramagnetic resonance spectrometer, for free radicals and transition metals detection
* ESI triple quadrupole mass spectrometer
* Three spectrophotometers and one fluorometer with temperature control
* Luminometer
* Extensive purification and analytical chemistry facilities (4 HPLCs, oxygen/nitric oxide sensors, etc)
* Hipoxic chamber
* Fluorescence microscopy